Cell Line Construction

Construction of a stable cell line usually takes 3 steps.

Cell line construction.

We have titrated the amount required for our hela cell lines and this can be a starting point for other cell lines. Stable cell line construction service. The health of the the target cell line is critical for obtaining accurate results. And 2 inducible reporter cell lines in which reporters are inducibly.

Methods to generate stable cell lines. The first step in the process is the isolation of single viable cells. Cell line development requires the discovery of single cell derived clones that produce high and consistent levels of the target therapeutic protein. Delivery of the target gene into the host cell screening of stable expression clones and stability test.

The development of a stable cell line is time consuming and complicated. To create stable cell lines is a key method in reducing the production costs of biopharmaceuticals. We do this for both gs system cell lines and cell lines brought to us by our customers. Limiting dilution is a technique that relies on statistical probability but is time consuming.

Stable cell line construction for protein antibody production. According to the upstream regulator of the reporter gene reporter cell lines can be further divided into two groups. 1 constitutive reporter cell lines in which reporters are constitutively expressed under the control of a constitutive strong promoter such as cmv promoter. Selection antibiotic e g puromycin microcentrifuge tubes.

Infection efficiency will vary between cell lines. Over time this will select for cells that have stably incorporated the gfp plasmid into their genomic dna. Lenti x 293t cells or alternative cell lines target cell line any human cancer cell line titered lentivirus containing your sequence of interest. Check the cells for mycoplasma regularly.

When large amount of recombinant protein is required stable expression line construction is recommended for reproducible and cost effective protein production. Biologics international corp bic owns highly qualified experts and decades of experience in upstream bio pharmaceutical development providing you with stable cell line development services. Do not over or under grow your cells. Do not add penicillin streptomycin to the media.

A mixed population of drug resistant cells can be used directly for experimental analysis with the advantage of generating fast results but also the disadvantage of dealing with an undefined and. With a proven track record in cell culture we offer a complete range of development services to establish a cell line and process that meet your needs and are suitable for manufacturing product for clinical or commercial supply. We use a modified frt flp in recombinase system to produce human proteins in cho cells and 293 cells for stable expression.