Cell Line Authentication Kit

To show the utility of the cla identifiler plus kit and seqstudio instrument for cell line authentication we analyzed dna from cultures of five different commonly used cell lines.

Cell line authentication kit.

The current recommended method for cell line authentication is short tandem repeat str profiling. The impact of cell line contamination. This service includes a collection kit dna collection card postage paid return envelope and certificate of analysis. The number of repeats at each.

Authentication of a cell line is the sum of the process by which a line s identity is verified and shown to be free of contamination from other cell lines and microbes. Standardization of str profiling guidelines using multiple kits. Geneprint 10 coamplifies and detects nine human loci th01 tpox vwa amelogenin csf1po d16s539 d7s820 d13s317 d5s818 and d21s11 these loci collectively provide a genetic profile with a random match. Str profiling aids in the detection of misidentified cross contaminated or genetically drifted cells which invalidate research results.

The profiling kits are only suitable for cell lines of human origin. Briefly gdna was purified from cell pellets using the invitrogen recoverall total nucleic acid isolation kit for formalin fixed paraffin embedded ffpe samples. This is the same technology used by the fbi for human dna identification testing hall explains. The geneprint 10 system provides a convenient str based method for cell line identity confirmation and for detection of intraspecies contamination in cultured human cell lines.

Cell line authentication kits cross contaminated or misidentified cell lines can ruin experiments leading to loss of precious time and funding. The idexx bioanalytics cell line authentication guide has the answers. The test utilizes primers that pick up repeated segments of dna that are typically 2 6 base pairs in length. Within the past five years a handful of publications have been retracted due to invalidated use of certain cell lines.

Further the results can vary depending on the history of the cell line and the type of media used for cell growth. Using standardized techniques authentication enables communication among all users about the resource and ensures valid reproducible experimental results. Grcf dna services offers cell line authentication analysis following ansi atcc asn 0002 2011 authentication of human cell lines. Mouse cell line authentication possible contamination is detected by one mouse gender marker two human markers and a 12 plex panel including 9 mouse tetranucleotide microsatellites.